The transcription of the main gene associated with Treacher–Collins syndrome (TCOF1) is regulated by G-quadruplexes and cellular nucleic acid binding protein (CNBP)

Treacle ribosome biogenesis factor 1 (TCOF1) is responsible for about 80% of mandibular dysostosis (MD) cases. We have formerly identified a correlation between TCOF1 and CNBP (CCHC-type zinc finger nucleic acid binding protein) expression in human mesenchymal cells. Given the established role of CNBP in gene regulation during rostral development, we explored the potential for CNBP to modulate TCOF1 transcription. Computational analysis for CNBP binding sites (CNBP-BSs) in the TCOF1 promoter revealed several putative binding sites, two of which (Hs791 and Hs2160) overlap with putative G-quadruplex (G4) sequences (PQSs). We validated the folding of these PQSs measuring circular dichroism and fluorescence of appropriate synthetic oligonucleotides. In vitro studies confirmed binding of purified CNBP to the target PQSs (both folded as G4 and unfolded) with Kd values in the nM range. ChIP assays conducted in HeLa cells chromatin detected the CNBP binding to TCOF1 promoter. Transient transfections of HEK293 cells revealed that Hs2160 cloned upstream SV40 promoter increased transcription of downstream firefly luciferase reporter gene. We also detected a CNBP-BS and PQS (Dr2393) in the zebrafish TCOF1 orthologue promoter (nolc1). Disrupting this G4 in zebrafish embryos by microinjecting DNA antisense oligonucleotides complementary to Dr2393 reduced the transcription of nolc1 and recapitulated the craniofacial anomalies characteristic of Treacher Collins Syndrome. Both cnbp overexpression and Morpholino-mediated knockdown in zebrafish induced nolc1 transcription. These results suggest that CNBP modulates the transcriptional expression of TCOF1 through a mechanism involving G-quadruplex folding/unfolding, and that this regulation is active in vertebrates as distantly related as bony fish and humans. These findings may have implications for understanding and treating MD.

activity of CNBP has been associated with its ability to bind and unfold G4s 9,26 (Fig. 1B) we next asked if putative G-quadruplex sequences (PQS) are present in the TCOF1 promoter.PQS-search engine QGRS Mapper 27 and G4Hunter algorithms identified two PQS (Fig. 1C), both sequences having a high potential to fold into threetetrads G4s as indicated by scores provided by both programs (Fig. 1D).Interestingly, PQS Hs791 and Hs2160 (named according their distance in bp to the TSS of TCOF1) overlap with two potential CNBP-BS previously defined (Fig. 1C).
In order to confirm the ability of Hs791 and Hs2160 to fold into G4 structures, synthetic single-stranded oligodeoxyribonucleotides (Supplementary Table S1) representing the two PQSs were analyzed by DNA intrinsic fluorescence and circular dichroism, two powerful spectroscopic methods used to characterize G4 formation 28,29 .In the presence of K + , both oligonucleotides showed an increased fluorescence signal (Fig. 2A, left panels), indicative of G4 formation 28 , with a most pronounced shift for Hs791 (left top panel) compared to Hs2160 oligonucleotide (left, bottom panel).The folding of both sequences into a G4 structure was confirmed by CD analyses.Both oligonucleotides fold into parallel G4s in the presence of K + , as indicated by the positive CD signal at ~ 260 nm and the negative peak at ~ 240 nm.For Hs2160, low K + concentration (1 mM) was enough to induce a strong CD signal, which was comparable to the signal obtained at 100 mM K + .In contrast, Hs791 required 20 mM KCl to induce a CD spectrum comparable to the one obtained at 100 mM, suggesting lower stability or propensity of Hs791 to fold into a G4 structure.In order to confirm these data, we assessed the stability of G4s by monitoring CD ellipticity at 260 nm during thermal denaturation (Fig. 2B).With a Tm of 62.7 °C Hs2160 shows a stronger stability than Hs791 (Tm 46.3 °C) even though the G4 in the Hs791 sequence was folded at higher K + concentration (20 mM) than that used for folding the G4 of the Hs2160 sequence (1 mM).Altogether, in vitro analyses of Hs2160 and Hs791 clearly show that both sequences fold into parallel G4 structures in the presence of K + with Hs2160 adopting a more stable G4 structure compared to the Hs791 sequence.
Next, to assess CNBP binding to PQSs/G4s present on the TCOF1 promoter, we performed EMSAs studies with the recombinant human CNBP protein 9,26,30 .Hs791 and Hs2160 sequences were previously folded either in the presence of 100 mM K + (folded G4) or in the presence of 100 mM Li + (unfolded sequences).The Table included in Fig. 2C reports the apparent K d values of interaction of CNBP with both Hs791 and Hs2160 sequences, in K + or Li + conditions (see also Suppl.Figs.S1 and S2).K d values obtained with both sequences show that CNBP protein binds to both folded and unfolded sequences with not statistical differences.
To demonstrate the ability of CNBP to bind both Hs791 and Hs2160 sequences in cells we performed chromosome immunoprecipitation (ChIP) assays using a stable HeLa cell line expressing CNBP fused to eGFP protein as previously described in David et al. 9 .After immunoprecipitation we observed a significant enrichment

5'-G-A-G-G-G-G-G-A-G-G-G-G-G-G
Asterisks indicate the six more conserved guanine residues as defined by the authors.(B) PQS consensus and diagram of G4 folding.Guanine-rich sequences capable of forming canonical G-quadruplexes consists in four tracts with 2-5 Guanine (orange), and between them, loops of variable nucleotides with different lengths (black).These kinds of sequences can fold into intramolecular stacked G-tetrads stabilized by coordination with monovalent cations.(C) Scheme of the human TCOF1 EPR (the region between the transcription start site (TSS, indicated with an arrow and + 1) and 5000 bp upstream) detailing the CNBP binding sites (CNBP-BS, green arrowheads) and PQSs (yellow diamonds).The 5′ untranslated region (5′UTR) is indicated by a black box.Below, the table shows the sequences (named according to their distance in bp to the TSS and with the guanine tetrads probably involved G4 formation marked in orange) and coordinates of each PQS that overlapped with CNBP binding sites found in the TCOF1 promoter.Also included are the results (scores) of web-based G4 predicting algorithms, QGRS-Mapper and G4Hunter.
of genomic DNA containing Hs791 and Hs2160 sequences (~8-fold) relative to IgG ChIP control (Fig. 2D) while the promoter region of GAPDH was not enriched in CNBP-eGFP ChIP assays, indicating that the binding of CNBP to the promoter region of TCOF1 gene was specific.Altogether, these results show that CNBP protein binds, in vitro and in HeLa cells, two G4 forming sequences present on the TCOF1 promoter.

G4s forming sequences present on the TCOF1 gene regulates transcription
To assess the capacity of G4 structures most likely present on the TCOF1 promoter to regulate transcription in cells we performed luciferase assays.Hs791 and Hs2160 sequences were cloned (following their genomic orientation) upstream to the basal SV40 promoter of the pGL3 vector.Control plasmids with guanines replaced by adenines to hinder G4 formation were also constructed (Suppl.Table S1) and the inability of modified sequences to fold into G4s was demonstrated by CD (Suppl.Fig. S3).Luciferase assays showed that the expression controlled by Hs2160, but not by Hs791, was significantly higher than that detected from the basal SV40 promoter (Fig. 3A) indicating a positive impact of the G4 forming sequence Hs2160 on transcription.Reinforcing this finding, luciferase expression from the mutated version of Hs2160 was found significantly lower than the wildtype Hs2160 PQS, with similar levels to those obtained with the empty vector (EV), confirming that the G4 forming sequence Hs2160 regulates the activity of the unrelated SV40 promoter.In addition, the luciferase approach failed to demonstrate any significant role of the Hs791 sequence on transcription.
To further explore the impact of G4 structures on endogenous TCOF1 transcription we performed RT-qPCR analysis of cells treated with different concentrations of the well-known G4 inducer and stabilizer Pyridostatin (PDS) [31][32][33] .Incubation with PDS 10 µM for 15 h led to a significant increase in TCOF1 mRNA level (Fig. 3B).In line with data from luciferase experiments, results support the positive role of G4 structures on TCOF1 transcription.SEM values obtained in EMSAs for each probe in both folding states.In all cases 3 independent repeats were performed for each probe and folding state.(D) ChIP assays performed on CNBP-eGFP expressing HeLa cells using anti-GFP or anti-IgG control antibodies.Bars represent the average enrichment of qPCR-amplified Hs2160 and Hs791 sequences in immunoprecipitated chromatin with anti-GFP antibody or with control antibody.GAPDH was used as amplification control (gene not regulated by CNBP).In all cases, bars represent the mean ± SD of three independent biological replicates.***P < 0.001, NS not significant, t test.www.nature.com/scientificreports/

CNBP unfolds the G4 structures formed by sequences present on the TCOF1 promoter
Works from several groups including ours have recently reported that the impact of CNBP on transcription could be related to its ability to unfold G4 structures present on promoters 9,[34][35][36] .From these findings we evaluated the influence of CNBP on parallel G4s formed by the PQSs present on the TCOF1 EPR.CD spectra of Hs2160 G4 structure showed a marked reduction and distortion of the characteristic ellipticity peaks, resulting from G4 folding, in the presence of 1:1 molar ratio of CNBP (Fig. 4A).In the same conditions, BSA, included as control did not significantly affect the spectrum.In line with that result, melting temperature of Hs2160 G4 monitored by ellipticity at 260 nm decreased by ~ 13 °C in the presence of CNBP (Fig. 4B).We also observed that CNBP induced a broader unfolding transition as displayed by the continuous decline of the ellipticity signal over a long temperature range.These results suggest that CNBP favors Hs2160 G4 unfolding and destabilization.On the contrary, the presence of CNBP did not affect Hs791 CD spectra nor melting temperature any differently from BSA (Fig. 4C,D), suggesting that CNBP has no specific action on Hs791 G4 folding.These results suggest that although both Hs2160 and Hs791 harbor CNBP binding sites, only Hs2160 is a stable G4 susceptible to be specifically unfolded by CNBP.

The EPR of the TCOF1 orthologous in zebrafish contains a PQS with transcriptional modulation capability
Results described above strongly suggest that the endogenous expression of TCOF1 is transcriptionally regulated by G4 in HEK293 cells, and that CNBP could act on TCOF1 transcription through its ability to unfold the G4 structure most likely formed in vivo by Hs2160 PQS.However, as immortal cultured cells might not be the proper physiological model to assess the role of CNBP in TCOF1 transcription control, we test that hypothesis in a model that is more attuned to the physiological context in which we speculate this regulation takes place: early embryo development.
It has been recently shown that several developmentally regulated genes contain evolutionary conserved G4s controlling transcription (e.g.noggin3; the orthologous of human NOG in zebrafish; col2a1a, the orthologous of human COL2A1) 20 .Identically to the approach adopted for TCOF1 EPR, we performed informatics analyses of EPRs of TCOF1 orthologues from Danio rerio (Fig. 5A) and other species (Suppl.Fig S4).Between seven to nine CNBP-BS were detected in warm-blooded species, including a remarkable preservation of the sites with Pan troglodytes.Noteworthy, the EPR of D. rerio gene contains the lower number of CNBP-CBS.
In all EPRs analyzed, the region between − 2000 and − 3500 from the TSSs presents an overlapping CNBP-BS PQS sequence.In zebrafish the sequence is located 2393 bp upstream the TSS (Dr2393, see in Fig. 5A the sequence and coordinates of this site, and the results obtained with the algorithms) and can adopt a two plateau G4 as predicted by QGRS Mapper and G4 Hunter algorithm.To further characterize the Dr2393 sequence we performed CD and intrinsic fluorescence spectroscopy.Both techniques showed that Dr2393 was able to fold as a G4 in the presence of K + ions (Fig. 5B).Also, Fig. 5C shows that Dr2393 introduced upstream the SV40 promoter in pGL3 promoter vector was able to induce luciferase activity at values statistically different from the empty vector or the vector carrying a mutated version of Dr2393 unable to fold as G4 (Suppl.Table S1 and Suppl Fig. S5).In line with the human Hs2160 sequence, the presence of Dr2393 upstream the SV40 promoter enhanced the luciferase transcription.www.nature.com/scientificreports/

CNBP binds and unfolds the G4 Dr2393
Next, to test the capacity of CNBP to bind to Dr2393 and if G4 folding affected the binding affinity, we performed non-radioactive EMSAs.Obtained apparent K d values, in the nanomolar range (Fig. 6A; Suppl.Fig. S6), showed no statistically different differential binding.Furthermore, we also demonstrated the in vivo binding of CNBP to the PQS containing region in zebrafish by ChIP analysis using the anti-eGFP antibody on total extracts obtained from embryos overexpressing CNBP-eGFP (Fig. 6B).
The effect of CNBP on Dr2393 was analyzed in vitro by CD spectra and melting analyses.CD spectra showed a reduction in the G4-specific positive ellipticity peak (~ 260 nm) and a distortion of the negative ellipticity peak (~ 240 nm) when CNBP was added to the Dr2393 PQS folded as G4 using a CNBP:PQS molar ratio of 1:1 (Fig. 6C).The addition of BSA induced minor changes in the spectrum, suggesting a specific effect of CNBP.Melting analyses confirmed these results (Fig. 6D).The Tm of Dr2393 was 60.5 °C in the absence of either CNBP or BSA, indicating a rather stable G4.The addition of CNBP in equimolar ratio induced a decrease of the measured Tm to 53.8 °C (~ 7 °C) while a similar addition of BSA only shifted the Tm a couple of °C (Fig. 6D).Also, in an analogous manner to Hs2160, the temperature induced a wider transition along an extended range of temperatures.Data endorse that CNBP binds and unfolds in vitro the G4 likely formed in vivo in the promoter of the zebrafish TCOF1 orthologue in a similar way as described above for the Hs2160 present in the human EPR.

A G4 forming sequence promotes the transcription of the TCOF1 orthologous in zebrafish embryos
Although data presented above indicated that Dr2393 enhances transcription activity of reporter promoters, the actual impact of this G4 structure in vivo in their genomic contexts during development needed to be addressed.ASOs had been used in zebrafish embryos to test the role of G4s in col2a1, fdz5 and nog3 transcription 20 .The same strategy was adopted in silkworm to study the role of G4 on Acyl-CoA binding protein gene transcription 21 .So, we targeted the G4 structure in the nolc1 EPR with specific ASOs to block its formation.Figure 7A  strategy: when complementary oligonucleotides are present at high levels, DNA tends to form a double-stranded structure rather than a G4 structure.For in vitro analyses, Dr2393-G4 was folded in the presence of K + , incubated for two hours in the presence of either specific (Dr2393-ASO) or control (Ctrl-ASO) ASOs, and finally analyzed by polyacrylamide gel electrophoresis.The incubation with Dr2393-ASO (even at 1:1 molar ratio) led to the presence of a band corresponding to the duplex-DNA.Conversely, the incubation with Ctrl-ASO did not modify the electrophoretic mobility of the Dr2393-G4 (Fig. 7B).
Next, we analyzed the effect of abolishing G4 formation on the expression of the nolc1 gene in vivo during zebrafish embryonic development.We injected the different ASOs into one-cell staged embryos, allowed them to develop until 24 hpf, and the relative level of nolc1 mRNA was measured using RT-qPCR.The transcription of the nolc1 gene was significantly lower in embryos injected with Dr2393-ASO compared to embryos injected with the Ctrl-ASO (Fig. 7C).The amounts of injected ASOs were those that did not produce any effects on embryo mortality compared to non-injected embryos (Suppl.Fig. S7), excluding transcriptional modifications related to embryos death.
In zebrafish, nolc1 knock-down induced by a translation-blocking Morpholino injection was shown to recapitulate most of features of TCS, such as decreased rRNA levels, increased cephalic neuroepithelial cell death and oxidative stress 6 .Moreover, cranial larval cartilages develop aberrantly, affecting first and second branchial arches derived structures 6 .In view of this, we evaluated if Dr2393-ASO injection mimics Morpholinoinduced knock-down phenotypes.One-cell stage embryos were injected with Dr2393-ASO or Ctrl-ASO and allowed to develop until 5 dpf.The larvae were fixed, and the cartilages were stained with Alcian Blue to visualize cranial cartilage morphology.The microinjection of Dr2393-ASO led to a significant increase in the number of larvae showing a phenotype related to nolc1 knock-down, which morphologic parameters were defined as described elsewhere 6,7 .Statistically significant changes were detected in all the parameters evaluated (Fig. 7D).In general terms, the heads of Dr2393-ASO injected larvae were shorter and the angles of the cartilages were higher, resulting in larvae with rounder heads.These results reinforce the idea of a decreased expression of www.nature.com/scientificreports/nolc1 due to the perturbation of Dr2393 folding impacting, as expected, in craniofacial cartilage development and phenocopying TCS (for a comparison with nolc1 Morpholino knock-down, please see Suppl.Fig. S8 and previous publications 6,7 ).

Cnbp levels in embryos modulate nolc1 transcriptional expression
We next evaluated the consequences of decreasing or increasing the CNBP levels on nolc1 expression during zebrafish embryo development.A translation-blocking Morpholino was employed to knock-down cnbp expression while the transgenic line XIa.Eefiai:cnbpa-EGFP, which expresses CNBP fused to GFP, was used to analyze the effect of overexpression.In both cases, the level of transcripts of nolc1 were measured by RT-qPCR in 24 hpf embryo extracts.With respect to controls, cnbp expression was reduced about 50% by the Morpholino injection; on the other hand, fluorescent embryos of the transgenic line XIa.Eefiai:cnbpa-EGFP exhibited cnbp levels ~ 70% higher than those measured in non-fluorescent specimens (Fig. 7E, left panel).Both CNBP knockdown and overexpression resulted in a statistically significant increase in nolc1 transcription levels, although the effect of knock-down appears to be greater than that of overexpression.

Discussion
While ample information exists regarding the molecular pathogenesis of TCS [37][38][39][40] , there has been relatively little focus on the cis-and/or trans-acting elements responsible for regulating the expression of the causative genes.Masotti et al. 41 studied the human TCOF1 promoter to investigate the influence of polymorphisms in the gene regulatory regions in TCS patients without mutations in coding regions.Transfection of reporter constructs into human HepG2 cells enabled the authors to identify a minimal promoter located around 150 bp upstream from the TSS and detect potential regulatory elements 1200 bp upstream of the TSS.However, regions beyond − 1200 of the TSS were not explored in that study.On the other hand, Shows and Shiang 42 observed that TCOF1 is expressed widely, but its levels are significantly higher in the developing neural crest compared to other tissues.Using Mus musculus promoter sequences, which are highly similar to those of H. sapiens, authors examined reporter constructs in two cell types: HEK293 (immortalized human embryonic kidney cells) and P19 (mouse embryonal carcinoma with pluripotent characteristics).A comparable minimal promoter, spanning from − 253 to + 47 bp, was identified in both cell lines as being responsible for strong and continuous transcription.Additionally, the upstream regions were suggested to enable cell type-specific repression.The authors focused their study on the mouse promoter region, examining up to − 1751 bp upstream.However, they acknowledged the presence of regulatory enhancer-like regions further upstream at approximately − 3, − 4.5, and − 6.5 kb that were not  characterized in that work.These two pioneering studies were conducted in cultured cells, examining regions close to the TSS.In order to expand our understanding of regulatory elements and achieve a contextualization of the phenomenon in a whole organism, in this study we present evidence pointing to a role for G4s as cisregulatory regions and CNBP as a trans-interacting factor in the transcription of TCOF1, both in cultured human cells and during zebrafish development.
Chambers et al. 19 reported the presence of at least 7 PQS (by computational methods) and between 27 and 17 potential G4s in the TCOF1 sequence (based on high-throughput sequencing in the absence or presence of K + and pyridostatin).In our study, we focused on the TCOF1 EPR sequence 5000 bp upstream of the TSS, and identified and confirmed two PQS with G4 folding propensity.Notably, one of these sequences, Hs2160, showed the ability to regulate the activity of the SV40 promoter in transiently transfected HEK293 cells, suggesting its potential regulatory role in vivo.This idea was further supported by the effect of PDS on the endogenous expression of TCOF1 in HEK293 cells.Even more, computational analysis of TCOF1 promoter sequences in other representative vertebrate species revealed multiple PQSs located between 3500 and 2000 bp upstream of the respective TSSs.In zebrafish, Dr2393 exhibited G4 folding and biochemical behavior in vitro similar to that observed for Hs2160.Furthermore, when cloned upstream of the SV40 promoter, Dr2393 demonstrated the ability to modulate luciferase expression in a manner consistent with Hs2160.The G4 forming sequences identified in the human and zebrafish TCOF1 EPRs are located 2160 bp and 2393 bp upstream of their respective TSS.The biochemical and regulatory behavior exhibited by both sequences, along with their shared location within the respective promoters, suggests that these G4s have been evolutionarily conserved in vertebrates.This conservation is likely due to their involvement in the transcriptional control of TCOF1.This hypothesis is partly supported by the data obtained in experiments where the formation of the G4 by Dr2393 PQS was specifically prevented in developing zebrafish through the injection of specific ASO.The ASO approach, which had been employed for evaluating the transcriptional role of G4s in zebrafish and other animals in vivo 20,23 , led to reduced transcription of nolc1, the TCOF1 zebrafish orthologue, along with aberrant cranial cartilages phenotypes similar to those ones reported when the nolc1 expression was knocked-down by specific Morpholino injection 6,7 .The absence of generalized unspecific phenotypic effects suggests that the obtained results were not owing to nonspecific or pleiotropic effects.Our results suggest that Dr2393 folded as G4 favors the transcription of nolc1 during the embryonic development of zebrafish.
Considering the varying numbers of PQSs and G4s identified by bioinformatics 15 , genome-wide mapping 19 , and chromatin immunoprecipitation 43 , it can be inferred that the formation of G4s is not solely determined by the presence of sequences with theoretical and biochemical potential.Instead, the physiological formation of G4s is regulated by cellular factors (such as chromatin compaction, helicase activity, and nucleic acid-binding factors) which dynamically shape G4s formation in vivo.Given these factors can significantly vary from one cell type to another, it is likely that cell lines may not be the most suitable models for assessing the physiological role of G4s.G4s are described as dynamic epigenetic marks predominantly found in nucleosome-depleted regulatory regions, correlating with actively transcribed genes 44 .According to Zhang et al. 45 , G4 formation can be induced remotely by downstream transcription events occurring thousands of base pairs away, as mechanical torsion propagates through the DNA double helix.Consequently, induced G4s have the potential to influence protein recognition and modulate transcription.Computational analysis of human TCOF1 gene indicated that, similar to other developmentally important genes in humans and other species 14 , several CNBP recognition sites are present in its promoter.This finding is not surprising since CNBP exhibits a similar spatiotemporal expression profile as TCOF1 and participates in the transcriptional control of various genes, including components of the Wnt signaling pathway, involved in skeletogenic cranial neural crest and proper craniofacial development 9,14,24 .Among these CNBP binding sites, two of them overlap with the PQSs Hs2160 and Hs791.Biophysical studies confirmed that both Hs2160 and Hs791 can fold as G4s.Hs791 sequence gives rise to a less stable G4 probably prone to unspecific unfolding prompted by any cellular condition.Our results support that although Hs791 may be a CNBP binding target, it is unlikely that a CNBP action relevant to cell biology is accomplished by regulating its G4 conformation.Instead, our results indicate that CNBP can bind to these sites in vivo, and in vitro experiments demonstrated that CNBP can also modulate the G4 folding state of Hs2160.Altogether, the results suggest that the transcription of TCOF1 could be regulated by CNBP through the destabilization of the G4 most likely formed in vivo by Hs2160.
As previously noted, our research group, along with others, has reported that CNBP plays a role in transcriptional control by unfolding G4s found on promoters 9,[34][35][36] .Various experimental approaches have indicated that the binding sequences of CNBP share the characteristic of containing G-enriched motifs, some of which may fold as G4s 8 .In fact, CNBP has been observed to bind G4s in DNA microarray experiments designed to study protein binding specificities across approximately 15,000 potential G4 structures.Interestingly, the disruption of G4 formation did not prevent CNBP from binding to the same sequences 46 .Additionally, CNBP has been identified as one of the proteins that interacts with both parallel and antiparallel G4s in a pull-down assay aimed at identifying cellular proteins that bind to G4s with well-defined topologies 47 .When the target sequences of CNBP fold as G4s, its G4-unfolding activity is driven by (i) a strong affinity for unfolded G-rich sequences, and (ii) a direct unwinding activity on the G4 core 8,9 .Consistent with its G4-unfolding activity, cells depleted of CNBP display an increased number of nuclear G4 foci, suggesting that CNBP acts as a global G4-unfolding protein 9 .Furthermore, CNBP has been identified as one of the G4-DNA binding sensitizers in the presence of small molecules that stabilize G4s 34 .CNBP G4-unfolding activity has been associated with the transcriptional activation of human c-MYC and KRAS oncogenes through the unfolding of repressive G4s in their promoters, as well as the transcriptional repression of human/zebrafish NOG/nog3 developmental genes by unfolding G4s with transcriptional enhancing activity in their promoters 9 .In this connection, and from the developmental biology perspective, it is worth noting that in zebrafish nolc1 depletion 6,7 appears to result in a milder craniofacial phenotype compared to cnbp depletion described elsewhere 8 .Despite potential technical differences, such as www.nature.com/scientificreports/variations in knock-down efficiency, which may lead to non-comparable levels of depletion, it's tempting to suggest that the depletion of CNBP, a multifaceted regulator with numerous targets 8 (now including nolc1 thanks to this manuscript) is, in the early stages of development, more detrimental than that of nolc1.Although further investigations are needed, this feature is consistent with the notion that CNBP may be a global regulator of embryonic development, as has been suggested 8 .
Our biophysical experiments support that CNBP functions unfolding the G4s most likely formed in vivo in TCOF1 promoter.So, from a mechanistic standpoint, our hypothesis (depicted in Fig. 8 for H. sapiens) is that under low levels of CNBP, Hs2160 (or Dr2393 in D. rerio) is more prone to be folded as a G4, favoring the transcription of TCOF1.This hypothesis is supported by the results obtained in the ASO microinjection experiments, which suggest that the unfolded state of the G4 represses nolc1 transcription.However, it is important to note that while this regulation may be directly mediated by the nucleic acid chaperone activity of CNBP 8,9 , it could also be due to the recruitment of additional protein factors.Although the hypothesis suggests that overexpression of CNBP would lead to a higher proportion of unfolded Dr2393 and lower levels of nolc1-mRNA, experiments performed in zebrafish specimens showed results contrary to what was expected.This could be attributed to the fact that, beyond the complexity of gene expression regulation in vertebrates, CNBP functions as a conserved pro-proliferative factor in eukaryotes (except in plants in which no orthologues of cnbp were reported) involved in both transcriptional and post-transcriptional regulation of multiple genes, affecting various targets 8 .In human mesenchymal cells obtained from control or TCS subjects, no significant differences were detected in the expression of TCOF1 and CNBP among the two cell types.However, a strong correlation between the expression of these genes was observed, suggesting a shared transcriptional regulation 6 .Increased cellular levels of CNBP may directly or indirectly affect downstream regulatory processes that contribute to maintaining appropriate levels of TCOF1 transcription.It is known that CNBP interacts with different cofactors to exert its modulatory actions on gene expression and that these actions can be contradictory 8,48,49 .Taking into account the above mentioned pro-proliferative actions of CNBP, a possible hypothesis to explain why its overexpression induces an increase in TCOF1 transcripts could be mediated by any of the other sites detected in the promoter or a direct or indirect mRNA stabilizing action (Fig. 8C).During embryonic development, the appropriate level of CNBP is critical for proper rostral development 14 .Altogether, under normal or nearly physiological levels of CNBP, these two genes exhibit a positive expression correlation.Nonetheless, when CNBP drops to levels insufficient for normal cranial development, a TCOF1 induction mechanism is revealed.As both CNBP and TCOF1 are involved in the multiple steps of RNA metabolism [50][51][52] , perhaps such a regulation can be interpreted as an attempt to keep cell translation homeostasis.
PQSs are commonly found in the promoters of oncogenes, while being less prevalent in tumor suppressor or housekeeping genes, suggesting an evolutionary selection of these elements based on gene function 53 .This distribution of PQSs has led to the design and characterization of various molecules belonging to distinct chemical families as G4 ligands 54 .Some of these ligands have been evaluated for their therapeutic potential in cancer [54][55][56] .Notably, TCOF1 has recently been mentioned as an oncogenic activator in hepatocellular carcinoma www.nature.com/scientificreports/(HCC) 57 .Considering the findings presented here, and acknowledging that extensive further research is required, it is possible to consider a future therapeutic approach utilizing a specific G4 ligand, such as a peptide-based ligand 58 , targeting Hs2160 to manage TCS symptoms or potentially address HCC.

TSS
In conclusion, our data suggest that at least one PQS located into the − 5000 bp of the TCOF1 promoter is able to fold as G4 and interact with CNBP to modulate gene expression.This phenomenon seems to be conserved among vertebrates, opening a gate to the study of gene regulation during craniofacial development and the exploration of alternative strategies for modulating TCS manifestations.

Bioinformatics
Gene promoter sequences were downloaded from Ensembl (http:// www.ensem bl.org/ index.html) genome versions GRCz11 (Danio rerio) and GRCh38 (Homo sapiens) [last accessed 23/6/2022 13:53 :16].Promoter regions (extended promoter region (EPR)) were arbitrary defined as the region spanning 5000 bp upstream from reported transcription start site (TSS).PQSs were searched using QGRS Mapper 27 and G4 Hunter algorithms 59 .In QGRS Mapper parameters were set to search for G4s formed by the stacking of at least two (D.rerio) or three guanines' tetrads (H.sapiens and other tetrapods) with loop sequence lengths spanning from 1 to 7 nucleotides and the results were fixed to leave out overlapping PQS.The QGRS-Mapper scoring method assigns higher scores (named G-score) to sequences that exhibit better potential as G4 candidates based on factors such as the number of possible stacked guanine tetrads and the similarity in length and size of the loops.In G4 Hunter, window size was 30 and step size was 10.Additional parameters were set to default.G4Hunter validates PQS ability to form G4 by taking into account G-richness (G in sequence) and skewness (G/C asymmetry with the complementary strand) of the PQS area and thus reduces false outcomes 59 .Putative CNBP DNA binding sequences (CNBP-BS) were searched in sequences using MEME/MAST (Motif Alignment and Search Tool) 60 and the CNBP-BS as reported elsewhere 24 .Parameters were: mast meme.xmlmart_export.txt-oc.-nostatus -remcorr -ev 10 -mev 1e−05.Only those sequences with E-value ≤ 1e−05 were kept for further studies.
Morpholino oligonucleotides (Suppl.Table S1) were obtained from Gene Tools (Philomath, OR, USA) and resuspended as indicated by the manufacturer.

Recombinant expression and purification of tag-free human CNBP
The pET-32a-TEV-CNBP plasmid expression and the tag-free human CNBP purification were performed following the guidelines detailed elsewhere 30 .Recombinant human CNBP was obtained in CNBP buffer (50 mM Tris-HCl pH 7.5; 300 mM NaCl; 1 mM DTT; 5 mM Imidazole and 0.1 mM ZnCl 2 ) at ~ 50 mg per litre of culture in a concentration of ~ 1 mg/ml.CNBP buffer was used in several in vitro assays as a control.

Circular dichroism (CD) and fluorescence spectroscopy
Oligonucleotides (Supplementary Table S1) were heated at 95 °C for 5 min at 2 μM concentration in 10 mM Tris-HCl, pH 7.5, with or without the addition of 1 to 100 mM KCl, and slowly cooled to room temperature over 2 h.CD spectra were recorded at 25 °C over a wavelength range of 220-320 nm with a Jasco-1500 spectropolarimeter (1 cm quartz cell, 100 nm/min scanning speed, 1 s response time, average of four scans).The spectral contribution of buffers, salts and drugs were appropriately subtracted by using the software supplied with the spectropolarimeter.In assays performed to evaluate CNBP effect on G4, prior to CD spectroscopy, CNBP or control protein dissolved in CNBP buffer were added to the solution of the folded G4 and incubated at 37 °C for 30 min.Then, CD spectra were recorded and analyzed as described elsewhere 20 .BSA (Promega) was used as control protein with no activity on G4s.Melting temperatures (T m ) were estimated according to Rachwal and Fox 61 .The CD melting curves were recorded by ellipticity measurements between 30 and 95 °C at the wavelength corresponding to the maximum observed at the initial temperature (20 °C) for positive band (≈ 264 nm), using the same parameters set for the spectra, except for 5 nm band width, a temperature increase speed of 1 °C/min, and a sampling interval of 0.1 °C.Data was analyzed in SigmaPlot 11.0 with a nonlinear least squares fitting procedure assuming a two-state transition of a monomer from a folded (G4) to an unfolded state with no change in heat capacity upon unfolding as described in David et al. 9 .
Oligonucleotides folded as G4s were also used for intrinsic fluorescence experiments.Fluorescence emission spectra were recorded at room temperature over a wavelength range of 300-480 (excitation wavelength 260 nm) in a Varian Cary Eclipse fluorescence spectrophotometer, essentially as described by Dao et al. 28 .

ASO PAGE assay
To test the disturbing effect of ASOs (anti-sense oligonucleotides) on G4s, G4-forming oligonucleotides (0.05 μM) were folded in 10 mM Tris-HCl, pH 7.5, 100 mM KCl by heating at 95 °C for 5 min and slowly cooling to room temperature over 4 h.After folding, the corresponding Dr2393-ASO or Ctrl-ASO were added to the folded oligonucleotides in 0.05 or 0.15 μM final concentration (1:1 and 1:3 molar ratio G4-forming oligonucleotide:ASO, respectively), and incubated for 1 h at 28 °C.Samples were resolved into 18% non-denaturing polyacrylamide

Electrophoretic mobility shift assays (EMSAs)
Non-radioactive EMSAs were performed as described previously 26 .Briefly, binding reactions were performed in 20 mM HEPES pH 8.0, 10 mM MgCl 2 , 1 mM EDTA, 0.5 µg/µl heparin, 1 mM DTT, 1 µg/µl BSA, and 10% glycerol.NaCl was omitted from the binding solution, and 100 mM KCl or LiCl were added, depending on the folding condition of the probe.DNA oligonucleotides used as probes were previously folded by thermal denaturation and slow renaturation in a buffer (10 mM Tris-HCl pH 8.0, 1 mM EDTA) in the presence of LiCl or KCl at concentrations of 100 mM, as indicated in figures.Probes were added to a final concentration of 0.75 µM.Final reaction volumes were 20 µl.Binding reactions were incubated for 30 min at 37 °C and then loaded in 15% polyacrylamide gels containing 5% glycerol in TBE 0.5×.After electrophoresis, gels were exposed for 10 min to SYBR Gold stain 62 .Fluorescence was subsequently visualized using a Gel Safe Imager S37102 UV Blue Light Trans illuminator (Invitrogen) and photographed using a Canon Powershot SX150 IS camera or registered in a Typhoon FLA 7000 Scanner using ImageQuant 5.2 software.The CNBP buffer was used for the CNBP dilutions and also supplemented in condition of absence of CNBP (0 µM).Apparent dissociation constants (K d ), representing the CNBP concentration that shifts 50% of the probe, were estimated as described in Suppl.Figs.S1 and S2.

Cell lines culture and methods
Human cell lines (Human embryonic kidney 293 cells (HEK293, ATCC CRL-1573), HeLa (ATCC CCL-2) and HeLa derivatives) were grown in DMEM media (Fisher Scientific) supplemented with 10% fetal bovine serum, at 37 °C under 5% CO 2 in a humidified atmosphere.HeLa CNBP-eGFP cells stably express the CNBP protein fused to enhanced green fluorescent protein (eGFP) and were maintained in DMEM media supplemented with 1 µg/ml puromycin.

Luciferase reporter experiments
Reporter experiments were performed as described elsewhere 20 .Briefly, plasmids containing the human PQSs (or their mutated versions impeding G-quadruplex formation) were generated by annealing oligonucleotides representing PQSs (or their mutated versions) with their complementary strands (Supplementary Table S1) and then cloned upstream the basal SV40 promoter by blunt-end ligation in SmaI digested pGL3-promoter vector plasmid (Promega).The plasmid with the zebrafish PQS (and mutated version) was obtained from amplifications using oligonucleotides containing the PQS or their complementary strands (Supplementary Table S1) together with sequences from the pGL3-promoter vector plasmid, and then inserted upstream the basal SV40 promoter by restriction-free cloning strategy 63 .Several clones were sequenced and those that contained the PQSs or their mutated versions in the same strand (coding or template) as they are found in genomes were used for transfections.HEK293 cells were cultured (250,000 cells/35 mm diameter dish) and the calcium phosphate method was used to transfect them as previously described 64 with 0.25 µg of unmodified pGL3-promoter vector plasmid (EV, empty vector) or reporter constructs.In addition, all dishes were co-transfected with 0.25 µg of pCMV-β-galactosidase (Promega) as a control for transfection efficiency.Luciferase activity was measured as described 20 , normalized to β-galactosidase activity and expressed as a ratio of luciferase/β-galactosidase.Finally, values determined for wild-type and mutated PQSs constructs were relativized to those for pGL3-promoter vector plasmid.Experiments were repeated three times.
Pyridostatin incubation experiments HEK293 cells were seeded in 35 mm plates at 150,000 cells per well.24 h later, culture medium was removed and replaced by new medium containing pyridostatin at 0, 1, 5 and 10 µM.Finally, 15 h after compound incubation, cells were processed for RNA extraction.Three independent incubation experiments were performed.

RNA extraction and RT-qPCR analyses
After compound incubation, cultured cells were recovered by trypsinization, washed with phosphate buffer saline (PBS) and the cell pellets used for total RNA extraction using TRIzol Reagent (Invitrogen) and reverse transcribed using MMLV reverse transcriptase (Promega).Relative mRNA levels were determined by the ∆∆Ct quantification method using the CFX Maestro software (Bio-Rad).GAPDH and HPRT mRNA levels were used as internal control housekeeping genes.The validity of the RTqPCR data was assured by following the MIQE guidelines 65 .

Zebrafish husbandry and methods
Adult zebrafish were maintained at 26.5 °C on a 14 h light/10 h dark cycle.Microinjection was performed on randomly separated sibling embryos at one-cell stage and embryos and larvae were staged according to development at 28 °C as described elsewhere 66 .ASO assays were performed essentially as described in David et al. 20 .Briefly, 5 nl of 5 ng/µl of each oligonucleotide (Supplementary Table S1) were injected, and embryos were raised up to the corresponding stage to perform RT-qPCR.For CNBP knock-down 6,7 , 5 nl containing 4 ng of CNBP translation blocking Morpholino or control Morpholino (see Supp.Table S1) were injected into the yolk.
For cnbp overexpression analysis, heterozygous Tg (XIa.Eefiai:cnbpa-EGFP) fish (http:// www.zfin.org) overexpressing cnbp fused to eGFP were crossed and embryos were selected according their fluorescence under stereoscopic MVX10 Olympus Microscope equipped with a MVXTV1XC Olympus digital camera.Green fluorescent embryos were grouped and as cnbp OE (cnbp overexpressing embryos) while non-fluorescent embryos were collected as control ones 6 .
Chromatin immunoprecipitation (ChIP) followed by PCR ChIP on 24-hpf transiently expressing CNBP-eGFP zebrafish embryos (by injection of one-cell embryos with 5 nl of 250 ng/µl of eGFP or Cnbp-eGFP capped-mRNA in KCl 100 mM) was performed as previously described 24 using anti-eGFP antibody (Abcam 290, Abcam).ChIP-PCR (20 μl) consisted of 0.2 μM of each primer (Supplementary Table S1), 2.5 mM MgCl 2 , 0.2 mM dNTPs, 0.5 U Taq DNA polymerase (Invitrogen) and 1 μl of each sample.After an initial denaturation step (94 °C for 10 min), 40 amplification cycles were performed, with each cycle consisting of a denaturing step of 30 s at 94 °C, an annealing step of 30 s at 63 °C and an extension step of 30 s at 68 °C, and a final extension step of 10 min at 68 °C.Actb2 promoter primers were assayed as specificity binding control.PCR products were analysed in 2% agarose gel and stained with Gel Green after running.

Reverse transcription and real-time quantitative PCR (RT-qPCR) assays
Total RNA from 24-hpf embryos was obtained using TRIzol Reagent (Invitrogen) following the manufacturer's instructions.Purified RNA was incubated with RQ1 DNAse (Promega) and oligo dT reverse transcribed with MMLV reverse transcriptase (Promega) to obtain cDNA.Quantification reactions were performed using three different RNA purifications from three independent microinjection experiments using an Eppendorf Realplex2 apparatus and SYBR green I (Invitrogen) chemistry.Each reaction tube (20 μl) consisted of 0.5× SYBR green I, 0.2 μM of each primer (Supplementary Table S1), 2.5 mM MgCl2, 0.2 mM dNTPs, 0.5 U Platinum Taq DNA polymerase (Invitrogen) and 2 μl of template/negative controls.Templates were 1:20 diluted cDNA samples.After an initial denaturation step (94 °C for 5 min), 40 amplification cycles were performed, with each cycle consisting of a denaturing step of 20 s at 94 °C, an annealing step of 30 s at 63 °C and an extension step of 30 s at 68 °C, and a final extension step of 10 min at 68 °C.

Zebrafish cartilages staining and image analysis
Four-day post-fertilization (dpf) larvae were fixed overnight in 4% (w⁄v) paraformaldehyde (PFA) in phosphatebuffered saline 1× (PBS) at 4 °C, washed and stained as described elsewhere 6 .Specific and control ASO microinjected embryos were observed with a MVX10 Olympus Microscope and recorded with a MVXTV1XC Olympus digital camera.Quantitative parameters were determined using the ImageJ software with a custommade plug-in as described in Gil Rosas et al. 7 (in detail 8 craniofacial measurements were scored: 1-Transversal Meckel length; 2-area of the inner triangle defined by the Meckel cartilage (Meckel area), 3-internal angle defined by the most anterior Meckel cartilage (Meckel angle); 4-length of ceratohyal cartilages, 5-internal angle defined by ceratohyal cartilages (ceratohyal angle), 6-length of palatoquadrate + hyosymplectic cartilages, 7-distance between the most anterior Meckel and lateral fins (cranial distance) and 8-distance between ceratohyal cartilages joint and lateral fins).

Statistical analysis
Experiments were independently repeated at least three times.Animals were randomly associated with the treatments and operator blinding was not done.The statistical analysis and graphing was performed using GraphPad Prism 8.01 (GraphPad, 2018) and Sigma Plot 12.0 (2018).The results are displayed as the mean value ± standard error of the mean (S.E.M.) unless otherwise indicated.Significant differences (P value < 0.05) between different groups were determined by unpaired t tests.The asterisks *, ** and *** indicate significance with P values less than 0.05, 0.01 and 0.001, respectively.

Ethics approval
Animal handling during this study was carried out in strict accordance with relevant local, national and international guidelines.Protocols were approved by the Committee on the Ethics of Animal Experiments of the Fac.de Cs.Bioquímicas y Farmacéuticas de la Universidad Nacional de Rosario (Record# 45026/2019; Resolution# 022/2020).This manuscript does not report on or involve any humans, human data or human tissue.

Figure 2 .
Figure 2. In vitro G4 folding assessment and CNBP binding of the Hs791 and Hs2160 PQSs.(A) DNA intrinsic fluorescence (in arbitrary units (a.u.), left panels) and CD (right panels) spectra of synthetic oligonucleotides representing the PQSs Hs791 (top) and Hs2160 (bottom).The potassium ions concentrations are indicated in the insets.(B) Thermal denaturation curves of synthetic oligonucleotides representing Hs791 (black) and Hs2160 (blue) obtained by monitoring the ellipticity at 260 nm as a function of temperature.Dots correspond to experimental data; lines are fits to a sigmoid function.Estimated melting temperatures (Tm) are informed in the plot.(C) Table reporting K d ± SEM values obtained in EMSAs for each probe in both folding states.In all cases 3 independent repeats were performed for each probe and folding state.(D) ChIP assays performed on CNBP-eGFP expressing HeLa cells using anti-GFP or anti-IgG control antibodies.Bars represent the average enrichment of qPCR-amplified Hs2160 and Hs791 sequences in immunoprecipitated chromatin with anti-GFP antibody or with control antibody.GAPDH was used as amplification control (gene not regulated by CNBP).In all cases, bars represent the mean ± SD of three independent biological replicates.***P < 0.001, NS not significant, t test.

Figure 3 .
Figure 3.Role of Hs791 and Hs2160 on transcriptional expression control.(A) Luciferase assay performed in HEK293 cells transfected with empty pGL3-promoter vector plasmid (EV, empty vector) or pGL3-promoter vector plasmid containing the wild type (wild-type PQS, black bars) or mutated (mutated PQS, grey bars) sequence of Hs791 and Hs2160 upstream the basal promoter SV40.Each bar represents the luciferase activity normalized to β-galactosidase activity and relativized to empty pGL3-promoter vector plasmid.Bars represent the mean of three independent experiments ± SEM. **P < 0.01, t test.(B) Effect of chemical stabilization of G4 on TCOF1 endogenous transcriptional expression in HEK293 cells.Pyridostatin (PDS), a well-known G4 stabilizer, was added to cells at the indicated concentrations for 15 h.RNA was isolated and TCOF1 expression was analyzed by RT-qPCR.Values are relativized to the non-incubated control (0 PDS).Bars represent the average of 3 independent experiments ± standard error of the mean.NS not significant, *P < 0.05, t test.

Figure 4 .
Figure 4.In vitro CNBP action on folded Hs791 and Hs2160.(A) CD spectra of 2 µM synthetic oligonucleotides representing Hs2160 folded as G4 in the absence of protein (black), in the presence of CNBP (1:1 ratio, red) or equimolar BSA as control (blue).(B) CD melting curves obtained for the synthetic oligonucleotides representing Hs2160 obtained in the absence of protein (black), in the presence of BSA as control (blue), or in presence of CNBP (red) at 1:1 molar ratio.Estimated melting temperatures (Tm) are informed in the plot.The dots are experimental values while lines indicate the non-lineal regression plot.(C) Similar to (A) but for folded Hs791.(D) Similar to (B) but for folded Hs791.

Figure 5 .
Figure5.Promoter sequence analysis of the TCOF1 ortholog in Zebrafish.(A) Scheme depicting the nolc1 (TCOF1 functional orthologue) promoter region showing the transcription start site (TSS), the detected CNBP-BS, (green arrowheads) and the PQSs (yellow diamonds).Below, the table shows the sequence (with the G tracts in color) and coordinates of the PQS that overlapped with CNBP-BS found (Dr2393) named according to the distance in bp to the TSS and the scores obtained with the two algorithms for PQS detection.The values obtained were above the threshold value defined by default for each algorithm, indicating a high probability of G4 formation.(B) CD spectra (left) and Intrinsic fluorescence spectroscopy (right) obtained for the synthetic oligonucleotides representing Dr2393 in absence or in presence of different concentrations of K + (shown in the inset).(C) Luciferase activity assay performed in HEK293 cells transfected with pGL3-promoter vector plasmid (EV, empty vector) or pGL3-promoter vector plasmid containing the wild type (Wild-Type PQS) or mutated (Mutant PQS) sequence of Dr2393 PQS cloned upstream the basal promoter SV40.Each bar represents the luciferase activity normalized to β-galactosidase activity and relativized to that for the empty pGL3-promoter vector plasmid.Bars represent means ± SEM, n = 3, **P < 0.01, t test.

Figure 6 .
Figure 6.CNBP binding and action over Dr2393.(A) Table summarizing the apparent Kd values (means ± SEM of 3 independent replicates for each state) obtained by EMSAs for the interaction of CNBP and Dr2393, folded as G4 and unfolded.(B) PCR experiment performed on ChIP samples from 24 hpf zebrafish embryos previously microinjected with mRNA coding for CNBP-eGFP.The arrow indicates the positive band in the detection of CNBP binding to Dr2393 in zebrafish embryos.ChIP (immunoprecipitation with anti-eGFP), Mock (immunoprecipitation with unrelated IgG), Input (total genomic DNA sample), C(-)PCR (negative PCR control).(C) CD spectra of 2 µM synthetic oligonucleotides representing Dr2393 folded as G4 in the absence of protein (black), in the presence of CNBP (1:1 ratio, red) or equimolar BSA as control (blue).(D) CD melting curves obtained for the synthetic oligonucleotides representing Dr2393 obtained in the absence of protein (black), in the presence of BSA as control (blue), or in presence of CNBP (red).Estimated melting temperatures (Tm) are informed in the plot.The dots are experimental values while lines indicate the non-lineal regression plot.

Figure 8 .
Figure 8. Diagram depicting the working model of TCOF1 transcriptional regulation by G4 and CNBP.Three different conditions showing the possible regulation of TCOF1.(I) Under physiological conditions, several CNBP binding sites in the TCOF1 promoter are occupied by CNBP, Hs2160 is unfolded and transcription proceeds normally.(II) Under high CNBP levels, probably all sites are occupied and extra CNBP may support TCOF1 expression by alternative ways (e.g. mRNA stabilization) either directly or indirectly.(III) Under low CNBP levels, less sites are occupied and Hs2160 has the chance to fold as a G4.Folded Hs2160 can recruit extra factors and induce TCOF1 transcription.

Table reporting K d
*P < 0.01, t test.(B) Effect of chemical stabilization of G4 on TCOF1 endogenous transcriptional expression in HEK293 cells.Pyridostatin (PDS), a well-known G4 stabilizer, was added to cells at the indicated concentrations for 15 h.RNA was isolated and TCOF1 expression was analyzed by RT-qPCR.Values are relativized to the non-incubated control (0 PDS).Bars represent the average of 3 independent experiments ± standard error of the mean.NS not significant, *P < 0.05, t test.
Twenty million HeLa CNBP-eGFP cells were fixed with 1% formaldehyde for 20 min at room temperature.selected promoter regions.For qPCR SsoFast EvaGreen Supermix (Bio-Rad) was used.ChIP-qPCR data were normalized to Input DNA to calculate the percentage of chromatin recovered by immunoprecipitation and to IgG control signals to evaluate the specific binding to target regions expressed as fold enrichment.